Rectal applications of ozone/oxygen mixture increase the antibody response of Hepatitis B virus to greater surface antigen in mice.
Borrego A., Zamora Z., MenŽndez S., L—pez L. and Hern‡ndez F. (Cuba)

Ozone therapy has become an useful therapy for the treatment of many pathological processes such as hepatitis, probably due to the viral inactivation by generated endoperoxides, as well as stimulation of cytokines release. Taking into account the immunomodulator effects of ozone therapy and the previous reports of its beneficial properties, we decided to study the effects of the ozone/oxygen mixture on antibodies response to hepatitis B related antigen. Balb C mice were treated with fifteen intra-rectal applications of ozone/oxygen at 0.25, 0.8, 2.2 and 4.4 mg/kg during intraperitoneal management of the greater surface antigen hepatitis B virus by a 0-7-14 days scheme at a dose of 2 µg per animal. Blood extractions were performed at 0-7-21 days by retroorbital punction. A sandwich direct enzyme linked immune sorbent assay of double antigen was performed in order to measure the specific antibody response. Ozone/oxygen applications induce a significant increase (p< 0.05) in antibody response to the immunogen. The stimulant effect of ozone was dose-dependent, but 2,2 mg/kg of ozone provoked the greater stimulation of the antibody response on day 21. Nevertheless, we have not found out a significant decrease in antibody response of animals treated with 4.4 mg/kg of ozone. Stimulant effect of ozone/oxygen mixture on the antibody response to greater surface antigen of Hepatitis B virus was demonstrated. It was corroborated that ozone/oxygen mixture can be useful on the recovery of B Hepatitis infection by increasing the antibody response to the virus, which favors the inactivation and elimination of it.