Ozone therapy in a cancer model (VX2 carcinoma: head and abdomen) in rabbits. A pilot study.
Schulz S., Sapundzhiev N., Dčnne A., Ramaswamy A., Bette M., Nčsing R., Moll R. and Werner J.A. (Germany)

Recently, Babior et al 2003 detected that ozone is endogenously produced in the human body which opened many questions about the role of ozone in biological systems. Thus exogenously applied ozone probably exhibits beneficial, non-beneficial or detrimental effects under physiological or pathophysiological conditions. We here report for the first time that exogenously applied ozone (O3/O2-pneumoperitoneum) exhibits a strong anticarcinogenic and antimetastasing effect on primary tumor cell inoculations (VX2 tumor cells) VX2-tumor and on the lymphogenic metastatic spread in rabbits. This treatment was without any visible side effects such as diarrhoe etc. The VX2-carcinoma model is a highly malignant Shope virus-induced squamous cell carcinoma with a high mortality rate (approx. 100 %) within 100 days of observation. We used this established aggressive tumor model with lymphogenic metastatic spread (bi-auricular model) of the primary local tumor and a local spread of peritoneal disseminated tumor cells (tumor nodules). In the ear model (n=5 rabbits), from initiated 8 solid aggressive primary tumors, 4 tumors dissappeared after a combined ozone treatment (local and systemic application for 5 days). Fifty percent of the ozone treated animals survived and they were tumor-free for > 500 days. All results from the macroscopic primary tumor size development, the pathohistolgy of the head and neck lymph nodes and lung metastasis after the tumor cell inoculations are presented in details. Furthermore, we present the effect of ozone treatment on the dissemination of tumor cells in the abdomen of rabbits. We think that ozone might be benefical agent in the treatment of these malignant tumors.

Experimental research of biological effects of ozone, radiation and chitosan in cancer animals.
Scherbatyuk T., Moskovceva O., Frolov V. (Russia)

The experiment was done on 580 white male rats. The neoplasia was modelled through Lymphosarcoma clone inoculation. The animals were subject to following actions: mono-radial influence, intra-abdominally oxygen influence and gamma-irradiation; intra-abdominally, ozone influence and gamma-irradiation; chitosan per se influence. Ozonated physiological saline was used at an ozone concentration of 400 Ág/L. State of free-radicals and antioxidant processes; glycometabolism; endotoxemia; phagocytosis; electrophoretic motility of erythrocytes and aggregation of erythrocytes were determined in blood and homogenates of tumour, lymphaden, and spleen tissues. Morphological and histological changes in tissues were observed. The new method (Shabalin, Shatichina, 1999) of morphological analysis of dehydrated blood plasma has been shown to be useful as a method of controlling efficacy of treatment in rats with limphosarcoma. The method being high sensitive, simple in performance. In the model of experimental oncology laboratory rats plus limphosarcoma it is shown, that ozone:

0.      In a complex of radial therapies increases antitumoral effect.

0.      In a complex of chitosan increases the antioxidant system of rats.

However in experiments concentration of ozone which causes a metastasis is revealed. Mechanisms of the effects are discussed.