OZONE APPLICATION IN NEOPLASIA TREATMENT OF EXPERIMENTAL ANIMALS. T. Scherbatyuk, C. Kontorschikova. Medical Academy, Nizhni Novgorod, Russia.
The aim of investigation was to study the ozone influence on some metabolic indexes of experimental animals affected by sarcoma-45. The experimental was done on 140 white male rats. The sarcoma was transplanted subcutaneously in the thing of the animals. 60 rats without ozonetherapy constituted the control group. Ozone therapy was carried out by introduction of ozonized saline solution (beginning 15th day of tumor cells implantation through 30 days) directly into the tumor. By the end of experiment samples of blood and tumor tissue were taken to study ozone effect on glycometabolism; lipid peroxidation (LP) system and phagcytosis activity of polymorphonuclear leukocytes (PAPL). There were determined the amount of glucose, lactate, pyruvate; molecular products of LP: diene and triene conjugates (DC, TC), Shiffbases; ceruloplasmin (Cp), reduced glutation (GSH), superoxide dismutase (SOD), catalase and PAPL. The received data revealed a valid decrease of lactate, pyruvate in the tumor, wile glucose amount in whole blood was higher compared with the intact. The latter is evidence that ozone abrogated glycopenia. It should be noted that ozone reliably decrease of SOD, catalase in tumor tissue. There was a slight rise in DC and TC readings. Blood analysis has shown that ozone valid increase of enzyme activity, Cp, GSH, PAPL but insignificantly change of LP products. According to the data it is possible to suggest that ozone may alter the metabolism in tumor and give carcinolytic effect.
OZONE CORRECTIVE EFFECT ON LIVER METABOLISM INDEXES IN EXPERIMENTAL ANIMALS WITH NEOPLASIA. C. Kontorschikova, T. Goncharova. Medical Academy, Nizhni Novgorod, Russia.
The results of the experiments to study ozone effect on phospholipid (PL) composition, lipid peroxidation activity (LP), and energetic state of liver cells in cancerogenesis dynamics are presented. Sarcoma-45 strain was reinoculated to adult white male rats. In two weeks of tumor growth the animals were infused ozonated saline. The first groups of rats received intraperitoneal infusions (1.5 mL) of ozonated saline for a week. The animals of the second group were intraperitoneally infused during the first week and directly into the tumor during the second week. Every species received 120 µg of ozone. Control group did not receive any treatment. With the tumor development liver examination revealed valid increase of primary (diene conjugates-DC) and final molecular LP products (Shiff bases-SB) and sphyngomyeline accumulation. It might have caused structural and functional state of cellular membranes and lowered down the energo-genetic processes in liver cells that was revealed in valid decrease of adenylic nucleotides. Ozone infusions caused a valid decrease of SB level by the end of the first week and brought DC content to normal one by the end of the second week. PL spectrum of the first group revealed prevalence of lysophosphatidyl choline and sphyngomueline in reduced phosphatidyl choline and phosphatidyl ethanolamine, making activation of oxidative processes evident in membrane. PL spectrum of the second group came completely to the initial level due to activation of antioxidant systems and phophollipases. By the end of the second week cellular energetic state was to be normalized. The latter is essential to restore basic liver functions that defend living organism against malignant tumors.