Effect of ozone therapy on the level of biomines in patients with rheumatic arthritis.
Kontorschikova C.N., Artemyeva E.G. and Zhuravlyeva N.V. (Russia)

The purpose of this article is to study the efficiency of ozone therapy in patients with rheumatic arthritis (RA), and first of all its effect on the dynamics of histamine, serotonin and catecholamine levels. Clinical observations in 140 patients with RA, at the age of 18 - 56 have been carried out. In 70 patients (control group) were administered intravenous infusions of ozonated saline solution (0,9 % NaCl), at an ozone concentration of 2 mg/L in conjunction with the conventional therapy. The other 70 patients (the comparative group) did not receive ozone therapy. Before and after the treatment, smears were taken from all the patients - imprints of periferal blood. To reveal catecholamines, serotonin and histamines fluorescence, histochemical technique with cytofluorometry in a fluorescence microscope, was used. Intensity of luminescence was measured in conditional units. Original blood tests of the RA patients showed elevated figures of histamine, serotonin, and catecholamines in erythrocytes, polymorphonuclear leucocytes, lymphocytes, thrombocytes, and plasma. In patients of the control group general condition after ozone therapy has improved, positive dynamics of the main parameters of rheumatoid process activity has been noted: ESR (erythrocyte sedimentation rate), levels of C-reactive protein, a2 - globulins, seromucoid, fibrinogen, hydroxiproline (p = 0.01), as well as albumine globulin index (p = 0.01). The reduction in the medication dosage for the patients of this group started from the 5 - 6th day. Dosages of nonsteroid preparations were cut on the average by 30 - 40 %. In half of the patients taking glucocorticoids, medications were withdrawn, in the others the intake reduced by 30 - 40 %. During ozone therapy more rapid regression of systemic manifestations has been observed (vasculitis, peripheral lymphodenopathy). The most significant changes of biogenic amine levels have been noted in polymorphonuclear leukocytes and lymphocytes. The reduction of all biogenic amines during ozone therapy was significantly more marked in the control group compared to the patients undergoing conventional therapy. Thus, positive effect of ozone on one of the pathological link of rheumatic arthritis has been clearly demonstrated.