OZ-PO-060: IN VITRO AND IN VIVO EFFECTS OF ORAL OLEOZON® ON GIARDIA LAMBLIA TROPHOZOITES. Damián Hernández (a), Frank Hernández (b), Zullyt Zamora (b), Maritza González (b), Odelsa Ancheta (c), Dinorah Torres (d) y Marta Bouza (d). (a) Instituto de Ciencias Básicas y Preclínicas "Victoria de Girón", (b) Centro de Investigaciones del Ozono, (c) Centro Nacional de Investigaciones Científicas, (d) Instituto de Medicina Tropical "Pedro Kouri", Cuba.
In the present paper in vitro and in vivo studies were carried out. Firstly, the in vitro inactivation capacity of OLEOZON® on Giardia lamblia trophozoite cultures was evaluated. Mean and maximum inactivation doses under controlled conditions were determined, and the effect of OLEOZON® on the Giardia lamblia ultrastructure was evaluated by electron microscopy. In the second part of this work, Giardia lamblia trophozoites inactivation was evaluated in inoculated gerbils treated with OLEOZON®. The effect of the treatment on several biochemical parameters in blood (glutathione peroxidase, catalase, glutathione S-transferase, reduced gluthatione, superoxide dismuthase, conjugated dienes, uric acid, reactive substances to thiobartituric acid and glucose 6-phosphate dehydrogenase) was measured. Results show that, depending on dose and microorganism concentration, OLEOZON® inactivates Giardia lamblia trophozoites by damaging the cell structure provoking the death of parasites. No significative change was observed in biochemical parameters. Under the experimental conditions of this study, OLEOZON® showed to be highly effective in inactivation and disinfecting of Giardia lamblia trophozoites and its effectiveness in this experimental model was similar to the obtained by metronidazol